Cis 9 trans 11 conjugated linoleic acid weight loss

Modeling of the data showed the exponential decay in 13C enrichment over time for both VA and cis-9, trans-11 CLA in milk fat. Enrichment was not detected in cis-9, trans-11 CLA for any plasma lipid fraction. Enrichment in VA was also detected in plasma TG, NEFA, PL, and MDG. Increased enrichment of 13C was determined using a 2-sample t test for each sample time compared with в€’24 h, with significance declared at P < 0.05. Lipid was methylated, converted to dimethyl disulfide and Diels-Alder adducts, and analyzed by GC-MS. Plasma lipid was separated into triacylglycerol (TG), cholesterol ester (CE), phospholipid (PL), nonesterified fatty acid (NEFA), and mono- and diacylglycerol (MDG) fractions. Milk and plasma lipid were extracted using chloroform:methanol. Blood and milk samples were taken frequently before and after VA infusion. Primiparous ruminally cannulated Holstein cows ( n = 3) were abomasally infused with 1.5 g of VA-1- 13C.

The utilization of 13C-labeled vaccenic acid (VA) by lactating dairy cows to synthesize cis-9, trans-11 conjugated linoleic acid (CLA) was investigated.

Cis 9 trans 11 conjugated linoleic acid weight loss